Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)

Authors

  • B. A. Lone Center of Research for Development, University of Kashmir, Srinagar-190006, Jammu and Kashmir, India
  • F. Ahmad Department of Zoology, University of Kashmir, Srinagar-190006, Jammu and Kashmir, India
  • H. Tak Department of Zoology, University of Kashmir, Srinagar-190006, Jammu and Kashmir, India
  • J. Hassan Govt. Degree College Bemina, Srinagar-190006, Jammu and Kashmir, India
  • M. Z. Chishti Center of Research for Development, University of Kashmir, Srinagar-190006, Jammu and Kashmir, India;
Abstract:

Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate the antibodytitre at different weeks post infection in experimental sheep. A comparison of plate ELISA on suspected fieldsera and faecal sample examination by floatation method revealed that 74 samples were found to be positive by ELISA but only ten by faecal examination. Sensitivity of plate ELISA was found to be 80.0%, whereas specificity was 21.42% indicating that this test is quite sensitive for clinical cases; an early diagnosis, however, lacks specificity.

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Journal title

volume 13  issue 1

pages  49- 53

publication date 2012-03-01

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